Publications

Full list of publications in peer-reviewed journals

Spatially‐Resolved Organoid Transfection by Porous Silicon‐Mediated Optoporation

C. Spiteri, V. Caprettini, Y. Wang, S. Dominguez‐Gil, M. Kaasalainen, C. Wang, D. A. Martella, S. McLennan, P. Vashisth, M. Gary‐Bobo, C. Nguyen, M. S. Bergholt, J‐O. Durand, F. Cunin, C. Chiappini
Advanced Materials 2024, 2407650

Label-free 3D molecular imaging of living tissues using Raman Spectral Projection Tomography

E. Stepula, A.R. Walther, M. Jensen, D. H. Mehrota, M.U. Yan, S.V Pedersen, V. Kumar, E. Gentleman, M.B. Albro, M.A.B. Hedegaard, M.S. Bergholt
Nature Communications 15, Article number: 7717 (2024)

A comparative study of analytical models of diffuse reflectance in homogeneous biological tissues: Gelatin-based phantoms and Monte Carlo experiments

A. Bahl, S. Segaud, Y. Xie, J. Shapey, M. S. Bergholt, T. Vercauteren
J. Biophotonics.2024;e202300536

Opto-lipidomics of Tissues

M. Jensen, S. Liu, E. Stepula, D. Martella, A. A. Birjandi, K. Farrell-Dillon, K. L. A. Chan, M. Parsons, C. Chiappini, S. J. Chapple, G. E. Mann, T. Vercauteren, V. Abbate,and M. S. Bergholt
Advanced Science, 2023, 2302962

Lithiated porous silicon nanowires stimulate periodontal regeneration

M. Kaasalainen , R. Zhang , P. Vashisth , A. Birjandi , Mark. S'ari , D. Martella, M. Isaacs , E. Makila , C. Wang , E. Moldenhauer , P. Clarke , A. Pinna, V. Caprettini , A. Morrell, Owen Addison , Professor Xuehui Zhang , M. S. Bergholt, K. Al-Jamal , A. Angelova-Volponi , J. Salonen , N. Hondow , Paul Sharpe , E. Gentleman, S. Mustfa, X. Zhang , D. Brauer, C. Chiappini,
Nature Communications (2024)15:487

SOL3D: Soft-lithography on 3D vat polymerised moulds for fast, versatile, and accessible high-resolution fabrication of customised multiscale cell culture devices with complex designs

C. Hagemann, M. C. D. Bailey, E. Carraro, V. M. Lionello, N. Khokhar, P. Suklai, C. Moreno-Gonzalez, K. O’Toole, G. Konstantinou, S. Joshi, E. Giagnorio, M. S. Bergholt, A. Imbert, Francesco S. Tedesco, A. Serio,
PLOS Biology (accepted)

Lightfield hyperspectral imaging in neuro-oncology surgery: an IDEAL 0 and 1 study

A Oscar MacCormac, Philip Noonan, Mirek Janatka, Conor C. Horgan, Anisha Bahl, Jianrong Qiu, Matthew Elliot1, Théo Trotouin, Jaco Jacobs, Sabina Patel, Mads S. Bergholt, Keyoumars Ashkan, Sebastien Ourselin, Michael Ebner, Tom Vercauteren, Jonathan Shapey
Front. Neurosci., 18 September 2023 Volume 17 - 2023

Synthetic white balancing for intra-operative hyperspectral imaging

Anisha Bahl, Conor C. Horgan, Mirek Janatka, Oscar J. MacCormac, Philip Noonan, Yijing Xie, Jianrong Qiu, Nicola Cavalcanti, Philipp Fürnstahl, Michael Ebner, Mads S. Bergholt, Jonathan Shapey, Tom Vercauteren
Journal of Medical Imaging, Vol. 10, Issue 4, 046001 (July 2023)

In Vivo Longitudinal Monitoring of Disease Progression in Inflammatory Arthritis Animal Models Using Raman Spectroscopy

A. R. Walther, E. Stepula, N. Ditzel, M. Kassem, M. S. Bergholt*, and Martin A. B. Hedegaard*
Analytical Chemistry 2023, 95, 7, 3720–3728

spectrai: A deep learning framework for spectral data

C. C. Horgan, M. S Bergholt
Journal of Spectral Imaging, a7, 11, 2022

Hybrid confocal Raman endomicroscopy for morpho-chemical tissue characterization

C. C. Horgan, M. Jensen, C. Chiappini, T. Vercauteren, R. Cook, M. S Bergholt
Biomedical Optics Express (accepted) 2022

High-throughput molecular imaging via deep learning enabled Raman spectroscopy

Conor C. Horgan, Magnus Jensen, Anika Nagelkerke, Jean-Phillipe St-Pierre, Tom Vercauteren, Molly M. Stevens, Mads S. Bergholt
Analytical Chemistry, 93(48) 15850-15860-2021.

Raman needle arthroscopy for in vivo molecular assessment of cartilage

Kimberly R. Kroupa, Man I Wu, Juncheng Zhang, Magnus Jensen, Wei Wong, Julie B. Engiles, Mark W. Grinstaff, Brian D. Snyder, Mads S. Bergholt, Michael B. Albro
Journal of Orthopaedic Research, 2021;1-11

Image-guided Raman spectroscopy probe-tracking for tumor margin delineation

Conor C. Horgan, Mads S. Bergholt, May Zaw Thin, Anika Nagelkerke, Robert Kennedy, Tammy L. Kalber, Daniel J. Stuckey, and Molly M. Stevens
J Biomedical Optics J 2021 26(3) 036002

Integrated photodynamic Raman theranostic system for cancer diagnosis, treatment, and post-treatment molecular monitoring

Conor C. Horgan, Mads S. Bergholt, Anika Nagelkerke, May Zaw Thin, Isaac J. Pence, Ulrike Kauscher, Tammy L. Kalber, Daniel J. Stuckey, Molly M. Stevens
Theranostics, Theranostics 2021; 11(4):2006-2019. doi:10.7150/thno.53031

In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Håkon Høgset, Conor C. Horgan, James P. K. Armstrong, Mads S. Bergholt, Vincenzo Torraca, Qu Chen, Timothy J. Keane, Laurence Bugeon, Margaret J. Dallman, Serge Mostowy, Molly M Stevens
Nature communications, https://doi.org/10.1038/s41467-020-19827-1 (2020)

Complementary techniques to analyse pericellular matrix formation by human MSC within hyaluronic acid hydrogels

Christoph Salzlechner, Anders Runge Walther, Sophie Schell, Nicholas Groth Merrild, Tabasom Haghighi, Isabella Huebscher, Gerhard Undt, Kathleen Fan, Mads Sylvest Bergholt, Martin A. B. Hedegaard and Eileen Gentleman
Materials Advances, https://doi.org/10.1039/D0MA00472C (2020)

Bioenergetic-active materials enhance tissue regeneration by modulating cellular metabolic state

Haoming Liu, Yingying Du, Jean-Philippe St-Pierre, Mads S. Bergholt, Hélène Autefage, Jianglin Wang, Mingle Cai1, Gaojie Yang, Molly M. Stevens and Shengmin Zhang
Science Advances, Vol. 6, no. 13, eaay7608 (2020)

Multiplexed polarized hypodermic Raman needle probe for biostructural analysis of articular cartilage

Magnus Jensen, Conor C. Horgan, Tom Vercauteren, Michael B. Albro, and Mads S. Bergholt
Optics Letters, Vol. 45, Issue 10, pp. 2890-2893 (2020)

Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling

Conor C. Horgan, Anika Nagelkerke, Thomas E. Whittaker, Valeria Nele, Lucia Massi, Ulrike Kauscher, Jelle Penders, Mads S. Bergholt, Steve R. Hoodd and Molly M. Stevens
Journal of Material Chemistry B, DOI: 10.1039/d0tb00620c

Surface enhanced Raman scattering artificial nose for high dimensionality fingerprinting

N. Kim, M. R. Thomas, M. S. Bergholt, I. J. Pence, H. Seoung, P. Charchar, N. Todorova, A. Nagelkerke, A. Belessiotis-Richards, D. J. Payne, A. Gelmi, I. Yarovsky, M. M. Stevens
Nature Communications, 207, 11, 2020

Raman spectroscopy: Guiding light for the extracellular matrix

M. S. Bergholt, A. Serio, M. B. Albro
Frontiers in Bioenginering and Biotechnology, 7, 1, 2019

Single particle automated Raman trapping analysis

J. Penders, I. J. Pence, C. Horgan, M. S. Bergholt, C. Wood, A. Najer, U. Kauscher, A. Nagelkerke, M. M. Stevens
Nature Communications, 9, 4256, 2018

Raman Spectroscopic Imaging for Quantification of Depth-Dependent and Local Heterogeneities in Native and Engineered Cartilage

M. B. Albro, M. S. Bergholt, J.P. St-Pierre , Á Vinals Guitart , H Zlotnick , E. Evita, M. M. Stevens
Nature Publishing Journals: Regenerative Medicine, 3, 3, 2018

Correlated Heterospectral Lipidomics for Biomolecular Profiling of Remyelination in Multiple Sclerosis

M. S. Bergholt, A. Serio, J. S. McKenzie, A. Boyd, R. F. Soares, J. Tillner, C. Chiappini, V. Wu, A. Dannhorn, Z. Takats, A Williams and M. M. Stevens
ACS Central Science, 4(1), 39–51, 2018

Raman spectroscopy imaging reveals interplay between atherosclerosis and medial calcification in human aorta

A Y. F. You, M S. Bergholt, J. P St-Pierre, A H. Chester, Magdi H. Yacoub, Sergio Bertazzo, Molly M |
Science Advances, 3(12),e1701156, 2017

Online Quantitative Monitoring of Live Cell Engineered Cartilage Growth Using Diffuse Fiber-Optic Raman Spectroscopy

M. S. Bergholt, M. B. Albro++ and M. M. Stevens
Biomaterials, 140, 128-137, 2017

Quantitative volumetric Raman imaging in three-dimenstional cell cultures

C. Kallepitis, M. S. Bergholt, M. M. Mazo, V. Leonardo, S. A. Maynard, S. C. Skaalure and M. M. Stevens
Nature Communications, 14843, 1-9, 2017

Raman Spectroscopy and Regenerative Medicine: A Review

K. Ember, M. Hoeve, S. McAughtrie, M. S. Bergholt, B. Dwyer, M. M. Stevens, Karen Faulds, S. J. Forbes, C. Campbell
Nature Publishing Journals: Regenerative Medicine, 12, 2017

Raman spectroscopy reveals new insight into zonal organisation of native and tissue-engineered articular cartilage

M. S. Bergholt, Jean-Philippe St-Pierre++, Giovanni S. Offeddu, Paresh A. Parmar, Michael B. Albro, Michelle L. Oyen & Molly M. Stevens
ACS Central Science, 2(12), 885-895, 2016

Quantitative multi-image analysis for biomedical Raman spectroscopic imaging

M. A. B. Hedegaard, M. S. Bergholt, M. S. Stevens
J Biophotonics, 1-9, 2016

Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy enhances real-time in vivo diagnosis of adenomatous polyps during colonoscopy

M. S. Bergholt, W. Zheng, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, A. Shabbir, Z. Huang |
Journal of Biophotonics, 9(4), 333-342, 2016

Characterising variability of in vivo Raman spectroscopic properties of different anatomical sites of normal colorectal tissue towards cancer diagnosis at colonoscopy

M. S. Bergholt, W. Zheng, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, A. Shabbir, Z. Huang
Analytical Chemistry, 87(2), 960-6, 2015

Fiber-optic confocal Raman spectroscopy for real-time in vivo diagnosis of dysplasia in Barrett's esophagus

M. S. Bergholt, W. Zheng, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, A. Shabbir, Z. Huang
Gastroenterolgy, 146(1), 27-32, 2014

Multivariate reference signals for improving quantitative analysis of fiber-optic tissue Raman spectroscopy

M. S. Bergholt, S. Duraipandian, W. Zheng, Z. Huang
Analytical Chemistry, 85(23), 11297-11303, 2013

Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, A. Shabbir, Z. Huang |
Journal of Biophotonics, 6(1), 49-59, 2013

Development of a multiplexing fingerprint and high wavenumber Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy

M. S. Bergholt, W. Zheng, Z. Huang
Journal of Biomedical Optics, 3, 030502, 2013

Development of a beveled fiber-optic confocal Raman endoscopic probe for enhancing in vivo epithelial tissue Raman measurements at endoscopy

J. Wang, M. S. Bergholt, W. Zheng, Z. Huang
Optics Letters, 3, 030502, 2013

Raman Endoscopy for Objective Diagnosis of Early Cancer in the Gastrointestinal System

M. S. Bergholt, W. Zheng, K. Y. Ho, M Teh, K. G. Yeoh, Z. Huang
Journal of Gastrointestinal & Digestive System, S1, 008, 2013

Real-time Raman spectroscopy for in vivo, online gastric cancer diagnosis during clinical endoscopic examination

S. Duraipandian, M. S. Bergholt, W. Zheng, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang |
Journal of Biomedical Optics, 17(8), 081418, 2012

In vivo, real-time, transnasal, image-guided Raman endoscopy: Defining spectral properties in the nasopharynx and larynx

M. S. Bergholt, K. Lin, W. Zheng, D. L. P. Lau, Z. Huang
Journal of Biomedical Optics, 17(7), 077002, 2012

Characterising variability in in vivo Raman spectroscopic properties of different anatomical sites of normal tissue in the oral cavity

M. S. Bergholt, W Zheng, Z. Huang
Journal of Raman Spectroscopy, 43(2), 255–262, 2012

Characterising variability in in vivo Raman spectra of different anatomical sites in the upper gastrointestinal tract toward cancer detection

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, Z. Huang
Journal of Biomedical Optics, 17(7), 077022, 2012

In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, Z. Huang |
International Journal of Cancer, 128(11), 2673-2680, 2011

In vivo diagnosis of esophageal cancer using image-guided Raman endoscopy and biomolecular modeling

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, Z. Huang
Technology in Cancer Research and Treatment, 10(2), 103-112, 2011

Combining near-infrared-excited autofluorescence and Raman spectroscopy improves in vivo diagnosis of gastric cancer

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. Bok Yan So, Z. Huang
Biosensors and Bioelectronics, 26(10), 4104-4110, 2011

Optical diagnosis of nasopharyngeal cancer using ultraviolet autofluorescence excitation-emission matrix spectroscopy and parallel factor analysis

B. Lin, M. S. Bergholt, D. Lau, Z. Huang
Analyst, 136(19), 3896-3903, 2011

Raman endoscopy for in vivo differentiation between benign and malignant ulcers in the stomach

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, Z. Huang
Analyst, 135(12), 3162-3169, 2010

In vivo early diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy

Z. Huang, M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh
Analyst, 134, 2123-2127, 2009

Quantification of C-Reactive protein in human blood plasma using near-infrared Raman spectroscopy

M. S. Bergholt, Soeren Hassing |